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. 2020 Sep 15;10:15079. doi: 10.1038/s41598-020-71941-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Representative photomicrographs of Goldner’s trichrome stained tibial growth plate obtained from 20-week OA rat and its intact tibiae. Epiphyseal plate (Ep), and cortical shell (Ct) were identified. Mineralized trabeculae (arrows) and marrow cells were stained green and red, respectively. Bars, 1 mm. Microstructural analysis of tibial metaphysis in OA rats and its intact leg at 20 weeks after OA induction as determined by bone histomorphometry. (B) trabecular bone volume normalized by tissue volume (BV/TV), (C) trabecular thickness (Tb.Th), (D) trabecular separation (Tb.Sp), (E) trabecular number (Tb.N), (F) osteoid volume (OV) normalized by bone volume (BV), (G) osteoid thickness (O.Th), (H) osteoclast surface (Oc.S) normalized by bone surface (BS), and (I) active erosion surface (aES) normalized by BS. Numbers of animals in each group are shown in parentheses. *P < 0.05 and **P < 0.01 vs. intact leg.