Table 1.

Metagenome information and results of DMSP-related gene searches in the metagenome data.

Samples	Depth (m)	Estimated % of cell with
		DMSP biosynthesis				DMSP cleavage								DMSP demethylation
		DsyB	MmtN	DSYB	TpMMT	DddP	DddQ	DddL	DddD	DddK	DddW	DddY	Alma1	DmdA
FL0a	0	0.78 ± 0.16	0.40 ± 0.18	0	0	6.48 ± 0.24	0.96 ± 0.14	0.64 ± 0.06	0.35 ± 0.16	0.26 ± 0.07	0.00	0.01c	0	35.73 ± 7.96
PA0a	0	0.89 ± 0.17	0.23 ± 0.01	0	0	6.05 ± 1.43	0.24 ± 0.17	1.29 ± 0.56	0.33 ± 0.05	0.29c	0.09c	0.00	0	12.73 ± 0.89
FL2000b	2000	0.43 ± 0.15	0.47 ± 0.16	0	0	5.34 ± 0.20	3.55 ± 0.63	1.09 ± 0.14	0.10 ± 0.06	0.00	0.00	0.13 ± 0.06	0	26.66 ± 7.17
FL4000a	4000	3.28 ± 1.88	0.33 ± 0.12	0	0	2.74 ± 0.87	1.79 ± 0.64	3.03 ± 0.41	0.24 ± 0.04	0.00	0.35c	0.12c	0	6.01 ± 0.99
PA4000a	4000	2.18 ± 0.02	0.65 ± 0.55	0	0	5.29 ± 1.85	0.35 ± 0.14	4.61 ± 1.91	0.34 ± 0.17	0.00	0.15 ± 0.05	0.03c	0	5.43 ± 3.16
FL8000b	8000	4.03 ± 1.92	1.22 ± 0.24	0	0	6.00 ± 0.61	0.71 ± 0.11	4.00 ± 1.19	1.61 ± 0.12	0.03c	0.00	0.06c	0	10.37 ± 0.88
FL9600	9600	2.23	0.36	0	0	1.03	0	1.40	0.19	0.00	0.00	0.00	0	1.65
FL10400	10,400	2.63	0.28	0	0	0.00	0	0.00	0.14	0.00	0.00	0.00	0	0.00
PA10400	10,400	2.26	0.00	0	0	0.24	0	0.64	0.30	0.00	0.00	0.00	0	0.00
FL10500	10,500	3.53	0.57	0	0	0.35	0.07	0.51	0.14	0.00	0.13	0.00	0	0.70
PA10500	10,500	3.58	0.28	0	0	0.12	0	0.42	0.34	0.00	0.12	0.00	0	0.42

FL free-living, PA particle-associated. Bacterial sequences were normalized to gene length. Estimated percentages were calculated using recA abundance. Data shown are means ± SD.

^aMean of two replicates, which are from different cruises but the same station (September 2016 and March 2017).

^bMean of two replicates, which are from the same cruise (March 2017).

^cValues from one cruise when another value is zero.