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. 2020 Sep 16;11:4666. doi: 10.1038/s41467-020-18379-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Edu incorporation in P7.5 Xinβ^KO and control hearts. DAPI marks nuclei, cTnT labels cardiomyocytes. Quantification in right panel. Scale bars = 100 µm. N = 4 and 5 biologically independent samples, respectively, *P < 0.05. b pH3 staining of P7.5 Xinβ^KO and control hearts. DAPI marks nuclei, α-actinin labels cardiomyocytes. Quantification in right panel. Scale bars = 40 µm. N = 4 and 3 biologically independent samples, respectively, *P < 0.05. c Heart morphology (left panel) and ventricle weight vs. tibial length ratio (right panel) of P7.5 Xinβ^KO and control hearts. Scale bars = 2 mm. N = 9 and 8 biologically independent samples, respectively,*P < 0.05. d Hematoxylin and eosin (red) and Fast Green (green) staining of p7.5 Xinβ^KO and control hearts. Scale bars = 1 mm. e AAV9-based CRISPR/Cas9 strategy to mutate the Xinβ gene in postnatal mouse hearts; f percent fractional shortening (FS) change in 4-week-old AAV-cTnT-Cre and AAV-Xinβ-sgRNA-treated mice. N = 13 and 12 biologically independent samples, respectively, *P < 0.05. g Morphology and immunostaining of isolated cardiomyocytes from hearts of adult Xinβ^KO and control mice. Scale bars = 50 µm.