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. 2020 Sep 16;11:4659. doi: 10.1038/s41467-020-18397-6

Fig. 3. GSK3008348-induced internalization and degradation of the αvβ6 integrin.

Fig. 3

Flow cytometry using NHBE cells showing a ligand-induced αvβ6 internalization triggered by GSK3008348, b the effect of chlorpromazine and filipin on ligand-induced αvβ6 internalization (mean ± SEM; n = 4–6; ANOVA with Tukey’s post test comparisons versus hβ6-PE or control), and c the kinetics of GSK3008348-induced αvβ6 internalization and return post-washout determined via flow cytometry in (mean ± SEM; n = 4). d Localization of αvβ6 integrin in NHBE cells post-GSK3008348 addition (β6 integrin (green) and nucleus (blue)) imaged by confocal microscopy to produce a 3D cell z-stack. Representative single cells (×50 magnification; scale bar = 50 µm) from four optical sections captured per condition with e the mean β6 marker intensity for optical sections shown (mean ± SEM from single experiment). f Flow cytometric analysis of the concentration-dependency of GSK3008348-induced αvβ6 internalization (mean ± SEM; n = 4). g The effect of the lysosomal degradation inhibitor chloroquine on GSK3008348-induced αvβ6 degradation in NHBE cells measured by high-content screening with example images and h quantification of αvβ6 via image analysis (×10 objective with average of five optical fields) (mean ± SEM; n = 5). Ligand-induced αvβ6 internalization in SAECs from i IPF (n = 2) and j non-diseased patients (n = 2) at 1 h. Source data are provided as a Source Data file.