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. 2020 Sep 16;11:4660. doi: 10.1038/s41467-020-18189-y

Fig. 2. Regionally specified GBM cells phenocopy intratumoral spatial identities.

Fig. 2

a IHC staining of mouse brains injected with edge or core 1051 GBM spheres for human mitochondria. Scale bar 2 mm. b IHC staining of mouse brains injected with edge or core 1051 GBM spheres for KRAS (upper), c-Myc (middle) and CHEK1 (lower). Scale bar 400um. c Representative immunofluorescence (IF) images of mouse brain slice culture seeded with edge 1051 (blue, yellow arrow) or core 1051 (green, white arrow) sphere cells and stained for Collagen IV to label blood vessels (red). Scale bar 50 µm. d IHC staining of a mouse brain co-injected with edge (unlabeled) and core (mCherry labeled) 1051 GBM spheres (ratio 1:1) for mCherry (lower, violet circle) and human mitochondria (upper, white circle). Scale bar 2 mm. e IF staining of the same samples as in “d” for human mitochondria (green), mCherry (red) and nucleus (blue). Scale bar 50 µm. f PCA of gene expression in edge, edge-like, core and core-like GBM sphere lines using set of 96-genes (32 each for proneural, mesenchymal and classical subtypes). g GSEA of core/core-like GBM spheres, compared to edge/edge-like GBM spheres. Gene sets shown include c-Myc, G2/M checkpoint, and KRAS-associated genes.