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. 2020 Sep 16;11:4660. doi: 10.1038/s41467-020-18189-y

Fig. 3. Intercellular signaling from core-like GBM cells provokes aggressiveness of their edge-like counterparts.

Fig. 3

a Table comparing overall survival (OS) and progression free survival (PFS) of GBM patients that underwent complete or non-complete resection (upper). The representative MRI image of pre- and postoperative brain, demonstrating residual enhancing lesions after surgery (lower). b Schema of the in vitro experiments with conditioned medium (CM). c In vitro cell growth assay of edge-like 1051 GBM spheres treated with/without CM from edge-like 1051 or core-like 267/1005/20 GBM spheres. Data are mean ± s.d., n = 4 independent samples per group.; ns, not significant; **p < 0.001 using one-way ANOVA followed by Tukey’s post-test. d Bioluminescence imaging (BLI) of mice intracranially injected with luciferase-labeled 1051 (n = 5 animals) or 157 (n = 3 animals) edge-like GBM spheres alone or together with unlabeled core-like GBM spheres (267, 1005) (ratio 95:5) (left). Quantification of BLI signal in mice (right). e In vitro cell viability assay of edge-like 157, 711, and 1051 GBM spheres pretreated with CM from core-like 267 GBM spheres and irradiated (IR) or left non-irradiated (non-IR). n = 4 independent samples per group. f Western blot (WB) for p65, phosphorylated p65 (p-p65) and CD44 using edge-like 157/711 GBM spheres treated with or without CM from core-like 267 GBM spheres. g IF staining for nucleus (blue), GFP (green) and CD44 (red) of mice brains bearing intracranial tumors developed from edge-like 1051 GBM spheres (labeled with GFP) alone, or co-injected with core-like 267 GBM spheres (unlabeled). Scale bar 20 µm. d–e Data are mean ± s.d. Significance was calculated by unpaired, two-tailed t-test with *p < 0.05; **p < 0.01; ***p < 0.001.