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. 2020 Sep 16;11:4660. doi: 10.1038/s41467-020-18189-y

Fig. 6. Inhibition of HDAC1 attenuates the intercellular signaling from core to edge GBM cells.

Fig. 6

a In vitro cell growth assay of edge-like 157 GBM spheres treated with CM from core-like 267 GBM spheres, which were infected with lentiviruses encoding shNT or shHDAC1. n = 4 independent samples per group. Data are mean ± s.d., ***p < 0.001 using one-way ANOVA followed by Tukey’s post-test. b In vitro cell viability assay of edge-like 157 GBM spheres pretreated as in “a” and subsequently irradiated. n = 4 independent samples per group. Data are mean ± s.d., ***p < 0.001 using one-way ANOVA followed by Tukey’s post-test. c Bioluminescence imaging of mice intracranially co-injected with luciferase-labeled edge-like 1051 GBM spheres and unlabeled shNT-infected or shHDAC1-infected core-like 267 GBM spheres (95:5 ratio) (left). Quantification of BLI signal in mice (right). n = 4 animals. d Same as in “c” for co-injection of luciferase-labeled edge-like 157 GBM spheres and unlabeled core-like 1005 GBM spheres, n = 4 animals. e IF staining for GFP (green), c-Myc (red), and nucleus (blue) of mice brains bearing intracranial tumors developed from co-injection of GFP-labeled edge-like 1051/157 GBM spheres and shNT-infected or shHDAC1-infected core-like 267/1005 GBM spheres. Scale bar 10 µm. f GSEA of shNT-infected core-like 267/28 GBM spheres, as compared to shHDAC1-infected spheres. Gene sets shown are c-Myc and G2/M checkpoint-associated genes. g Heatmap of RNA-seq data comparing expression of selected genes in core-like 267/28 GBM spheres infected with shNT or shHDAC1. CD109 indicated by red arrow. c, d Data are mean ± s.d. Significance was calculated by unpaired, two-tailed t-test with *p < 0.05; **p < 0.01.