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. 2020 Sep 16;10:15211. doi: 10.1038/s41598-020-72167-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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A weakening response in cellular stress response and TPS2 was identified as a lifespan marker. (a) Periods stimuli, every 6 h with 1-h stimuli of 0.4 M KCl for 72 h and (b) 1 h stimuli of 0.4 M KCl from 23 to 24 h. Response capacity I_r/T of TPS2 and residual lifespan showed a significant positive correlation (two sets of experiments contained 97 and 99 cells, respectively). The lilac column represents the moment of adding osmotic stimulation. (c) Characterization of internal noise during the lifespan of yeast cells. (d) The relationship between TPS2 production (solid line, linear fit) and the internal noise (more than 60 cells).