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. 2020 Sep 16;10:15211. doi: 10.1038/s41598-020-72167-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transcription factors remain equivalent during the aging process. (a) Transcription factors (HOG1 and MSN2) translocate into the nucleus in response to osmotic stimuli. (b) HOG1-GFP images at the indicated times. The orange dotted box represents the stimulation time. (c) In response to a step stimulus of 0.4 M KCl, dynamic curves of single-cell HOG1 and MSN2 nuclear enrichment. Averaged trace of single-cells is shown in dark (left). The schematic defines the amplitude, duration and integral (oblique line) of the nuclear translocation of transcription factors in a single-cell time trace (right). (d) Integral of TFs corresponding to the datasets described in (c). Boxes extend from the 25th to 75th percentiles [interquartile range (IQR)]; horizontal lines represent the median, and whiskers indicate the lowest and highest datum within 1.5 × IQR from the lower and upper quartiles, respectively.