Figure 2.

EBV-miR-BART10-5p and hsa-miR-18a Have a Crucial Role in NPC Angiogenesis

(A–C) BART10-5p and miR-18a were able to promote NPC angiogenesis individually in in vitro models (tube formation assay and CAM assay) (A and B) and in an in vivo model (Matrigel plug assay) (C). (E–G) Inhibition of BART10-5p and miR-18a were able to inhibit NPC angiogenesis individually in in vitro models (tube formation assay and CAM assay) (E and F) and in an in vivo model (Matrigel plug assay) (G). Furthermore, co-treatment with mimics/agomiRs inhibitor or antagomiRs of BART10-5p and miR-18a got a stronger response. Furthermore, co-treatment with BART10-5p and miR-18a got a stronger response. (C and G) Representative images (left) and hemoglobin quantification (right) of the Matrigel plug assay (n = 3, Student’s t test). (D and H) Expression of CD31, which represents MVD in Matrigel plug sections (n = 3, Student’s t test). Original magnification, ×200. All images were taken at ×200 original magnification. All bars and error bars stand for mean values and corresponding SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.