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. 2020 Sep 16;40(9):BSR20201366. doi: 10.1042/BSR20201366

Figure 2. Effects of piceatannol on the pyroptosis of macrophages after oxLDL challenge.

Figure 2

(A) qPCR analyses of the relative mRNA levels of caspase1, GSDMD, IL-18, IL-1β, and NLRP3 on RAW264.7 cells after piceatannol treatment. Gene expression was normalized to β-actin mRNA level. *P<0.05, **P<0.01, ***P<0.001. The experiments were repeated four times. (B–D) Representative Western blot analysis (B) and quantification (C,D) of caspase1, GSDMD, NLRP3, cleaved IL-18 and IL-1β protein on RAW364.7 cells after piceatannol administration. Protein expression was normalized to β-actin levels. *P<0.05, **P<0.01, ***P<0.001. The experiments were repeated three times. (E) Analysis of LDH release after piceatannol treatment. **P<0.01, ***P<0.001. The experiments were repeated four times. Abbreviation: qPCR, real-time PCR.