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. 2020 Sep 16;10:15160. doi: 10.1038/s41598-020-72122-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of EVs using NTA and TEM. (A) Representative graphs show NTA results of size and concentration of the EVs. (B) Representative TEM images, below each graph, show the isolated EVs from parental-EV, 4-1BBL-EV, and OX40L-EV, using the negative staining method. The NTA images were analyzed by the software NanoSight NTA 3.1 (URL: https://www.malvernpanalytical.com/en/support/product-support/nanosight-range/nanosight-ns300). The TEM images were acquired, analyzed, and processed by Gatan Digital Micrograph (GMS3) software (URL: https://www.gatan.com/products/tem-analysis/gatan-microscopy-suite-software).