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. 2020 Sep 16;10:15160. doi: 10.1038/s41598-020-72122-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Tumor-derived EVs induce T cell proliferation. CD4+ T cells were labeled with anti-CFSE, incubated with indicated EVs and analyzed by flow cytometry. (A) Representative histograms show T cell proliferation. (B) Graphic representation to consolidate the proliferation results of three independent experiments. (C) Proliferation index. Data expressed in (B) and (C) were analyzed by ANOVA with Dunnett post test (**p < 0.05). (–): only CD4+ T cells, Par-EV, parental EVs.