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. 2020 Sep 16;10:15160. doi: 10.1038/s41598-020-72122-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TNFSF-EVs inhibit FoxP3 expression in regulatory T cells. CD4+ T cells were treated with ITR induction cocktail and incubated with the indicated EV preparation. Cells were harvested, stained against FoxP3 and analyzed by flow cytometry. (A) 5 × 10⁹ particles/mL and (B) 2 × 10¹⁰ particles/mL. Three independent experiments were performed. One-way ANOVA statistical test followed by Dunnett’s test, *p < 0.05; mean and standard error.