REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-CD11c AF488 (Lineage)	BioLegend	301618; RRID: AB_439791
Anti-CD14 FITC (Lineage)	BD Bioscience	555397; RRID: AB_395798
Anti-CD19 FITC (Lineage)	BD Bioscience	560994; RRID: AB_10563406
Anti-CD3 AF488 (Lineage)	BioLegend	300320; RRID: AB_493691
Anti-CD4 FITC (Lineage)	BioLegend	317420; RRID: AB_571939
Anti-TCRgd AF488 (Lineage)	BioLegend	331208; RRID: AB_1575108
Anti-TCRab AF488 (Lineage)	BioLegend	306712; RRID: AB_528967
Anti-CD34 FITC (Lineage)	BioLegend	343604; RRID: AB_1732005
Anti-CD303 FITC (Lineage)	BioLegend	354208; RRID: AB_2561364
Anti-CD19 FITC (Lineage)	BD Bioscience	560994; RRID: AB_10563406
Anti-CD94 PerCp.Cy5.5	BD Bioscience	562361; RRID: AB_11152081
Anti-CD117 BV421	BioLegend	313216; RRID: AB_11148721
Anti-CD117 BV650	BioLegend	313221; RRID: AB_2562714
Anti-CD161 BV605	BioLegend	339915; RRID: AB_11142679
Anti-CD16 BV650	BioLegend	302042; RRID: AB_2563801
Anti-CD56 BV711	BioLegend	318336; RRID: AB_2562417
Anti-CD3 BV785	BioLegend	317330; RRID: AB_2563507
Anti-CD294 AF647	BD Bioscience	558042; RRID: AB_2112699
Anti-CD38 AF700	BioLegend	303516; RRID: AB_2072782
Anti-CD95 PE-CF594	BioLegend	305634; RRID: AB_2564221
Anti-CD127 Pe-Cy7	Beckman Coulter	A14934; RRID: AB_2534372
Anti-CD4 BUV496	BD Bioscience	564651; RRID: AB_2744422
Anti-PD-1 BV421	BD Bioscience	562516; RRID: AB_11153482
Anti-CD103 BV605	BioLegend	350218; RRID: AB_2564283
Anti-CD69 BV785	BioLegend	310932; RRID: AB_2563696
Anti-CD3 PE-CF594	BD Bioscience	562280; RRID: AB_11153674
Anti-CD366 (NKp44) PE-Cy5	Beckman Coulter	A66903; RRID: AB_2857937
Anti-CD8 BUV396	BD Bioscience	563795; RRID: AB_2722501
Anti-CD19 BUV496	BD Bioscience	564655; RRID: AB_2744311
Live/Dead Fixable Near-IR Dead Cell Stain Kit, 633nm	Invitrogen	L10119
Anti-IL-2 BV650	BD Bioscienceces	563467; RRID: AB_2738224
Anti-IL-4 FITC	BioLegend	500807; RRID: AB_315126
Anti-IL-5 APC	BioLegend	504305; RRID: AB_315329
Anti-IL-13 BV421	BioLegend	561158; RRID: AB_10561838
Anti-TNFa AFlour700	BD Bioscience	557996; RRID: AB_396978
Anti-IFNg PE-Cy7	BD Bioscience	557643; RRID: AB_396760
Anti-CD294 (CTRH2) PE-CF594	BD Bioscience	563501; RRID: AB_2738244
Anti-CD127 PE-Cy5	Beckman Coulter	A64617; RRID: AB_2833010
Biological Samples
Human peripheral blood mononuclear cells (PBMCs)	Human	Cohorts
Human tonsil mononuclear cells (TMCs)	Human	Cohorts
(See Table 1)
Chemicals, Peptides, and Recombinant Proteins
Maxima H-RT and Buffer	ThermoFisher Scientific	EP0751
dNTPs	New England Biolabs	N0447L
SUPERase∗In RNase inhibitor	ThermoFisher Scientific	AM2696
Betaine solution, 5M, PCR Reagent	Millipore Sigma	B0300-5VL
KAPA 2x HiFi HotStart PCR mix	Kapa Biosystems	KK2602
RNAClean XP	Beckman Coulter	A63987
AMPure XP	Beckman Coulter	A63881
Nextera XT Kit	Illumina, Inc	FC-131-1096
Oligonucleotides
SMART Oligo dT	IDT	/5Biosg/AAGCAGTGGTATCAACGCAGAGTAC(T)30VN
Template-Switching Oligo	IDT	AAGCAGTGGTATCAACGCAGAGTACATrGrGrG
SMART PCR Primer	IDT	AAGCAGTGGTATCAACGCAGAGT
Software and Algorithms
FlowJo	TreeStar	v9.9.6
Prism	GraphPad Software	v8.4.3
DESeq2	Li and Dewey, 2011	V1.18.1
Ingenuity Pathway Analysis	QIAGEN Inc.	Winter 2019 Release