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. 2020 Sep 3;11:541426. doi: 10.3389/fphar.2020.541426

Figure 2.

Figure 2

SalB treatment reversed the effect of pyroptosis related genes in ischemia-reperfusion (I/R) mice. (A–D) Western blot analysis of the active cleaved form of caspase-1 (Cl-casp1), proform of caspase-1 (Pro-casp1), active cleaved form of interleukin-1β (IL-1β) (Cl-IL-1β), proform of IL-1β (Pro-IL-1β), gasdermin D (GSDMD), GSDMD-N terminal segment (Cl-GSDMD). The data are presented as the mean ± SEM (n = 3), *P < 0.05, **P < 0.01 vs. sham group; #P < 0.05, ##P < 0.01 vs. I/R group. (E, F) Immunohistochemical staining and quantitative analysis for caspase-1 was shown (n = 6, magnification: 400×). (G, H) mRNA levels in renal tissues of IL-1β and TNF-α determined by real-time polymerase chain reaction (PCR). The data are means ± SEM (n = 6).*P < 0.05, **P < 0.01 vs. sham group; #P < 0.05, ##P < 0.01 vs. I/R group.