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. 2020 Sep 15;32(11):108141. doi: 10.1016/j.celrep.2020.108141

Figure 2.

Figure 2

Adipocyte-Specific GPS2 Deficiency in Mice Leads to an Impaired Insulin Secretion in Response to Glucose

(A) qRT-PCR analysis of GPS2 in eWAT from WT C57BL6/J under HFD feeding for 12 weeks (n = 18), classified into 2 groups: high GPS2 expression (n = 9) and low GPS2 expression (n = 9).

(B) Oral glucose tolerance test (OGTT) in WT C57BL6 after 12 weeks of HFD classified into 2 groups: high GPS2 expression (n = 9) and low GPS2 expression (n = 9).

(C) Measurements of insulin secretion during the OGTT in WT C57BL6/J after 12 weeks of HFD classified into 2 groups: high GPS2 expression and low GPS2 expression (high GPS2 n = 9 and low GPS2 n = 9).

(D) Glucose-stimulated insulin secretion calculated using the ratio of insulin secretion during OGTT at 15 min (T15) to basal (T0) in WT C57BL6/J after 12 weeks of HFD classified into 2 groups: high GPS2 expression and low GPS2 expression (high GPS2 n = 9 and low GPS2 n = 9).

(E) Fasting insulin concentration in WT and GPS2 AKO mice in 4 and 12 weeks of HFD (n = 5 to 6).

(F) Measurements of insulin secretion during the OGTT in WT and GPS2 AKO mice in 4 and 12 weeks of HFD (4 weeks HFD n = 6; 12 weeks HFD n = 5 to 6).

(G) Glucose-stimulated insulin secretion calculated using the ratio of insulin secretion during OGTT at 15 min (T15) to basal (T0) in WT and GPS2 AKO mice in 4 and 12 weeks of HFD (4 weeks HFD n = 6; 12 weeks HFD n = 5 to 6).

All data are represented as mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.