REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Monoclonal insulin antibody (Alexa 647 Fluor conjugated)	Cell signaling	Cat# C27C9; RRID: AB_2687822
Mac2 Monoclonal antibody	Cedarlane	Cat# CL8942AP; RRID: AB_10060357
In situ Cell death detection kit, TMR red	Sigma-Aldrich	Cat# 12156792910; RRID: N/A
KI67 Monoclonal antibody	e-bioscience	Cat# 13-5698-82; RRID: AB_2572794
Biological Samples
Mouse adipose tissue explants	This paper	N/A
Chemicals, Peptides, and Recombinant Proteins
Insulin (HUMALOG 100 unites/ml)	lilly	N/A
Insulin, Human Recombinant	Sigma	Cat#I-9278
Rosiglitazone	Sigma	Cat#R-2408
Iso-butyl-methylxanthine	Sigma	Cat#I5879-SG
Dexamethasone	Sigma	Cat#D1756
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP)	Sigma	Cat#C2920
Critical Commercial Assays
MULTIPLEX Adipocyte kit	Millipore,	Cat# MADCYMAG-72K
Ultrasensitive mouse/rat insulin kit	Meso scale discovery	Cat# K152B2C-3
M-MLV Reverse Transcriptase kit	invitrogen	Cat# 28025-021
RNeasy RNA Mini Kit	qiagen	Cat# 74136
Sybergreen	Eurogentec	Cat# RT-SY2X-03+WOUFL
Taquman	Promega	Cat# A6102
Deposited Data
Data files for RNA sequencing	NCBI Gene expression: https://www.ncbi.nlm.nih.gov/geo/	GEO: GSE111647
Cell lines
Primary pre-adipocytes	This paper	N/A
Isolated mouse islets	This paper	N/A
Experimental Models: Organisms/Strains
Mouse: Adipoq-Cre	Jackson Laboratory	010803
Mouse: Gps2flox/flox mice	Fan et al., 2016	Generated at Ozgene Pty, Ltd. (Bentley DC, Australia)
Mouse: c57bl6	The Jackson laboratory	000664
Oligonucleotides
See Table S2 for the list of Oligos	N/A	N/A
Software and Algorithms
FIJI	FIJI Software	https://fiji.sc/
Graphpad 7	GraphPad Software	https://www.graphpad.com/