Table 1.
Metabolic Outcomes from Villin Cre and Txnip Villin Cre Mice
| Metabolic Parameters | Villin Cre |
Txnip Villin Cre |
||
|---|---|---|---|---|
| RD (n = 4) | ERD (n = 4) | RD (n = 4) | ERD (n = 4) | |
| Fat mass (g) | 2.9 ± 0.5 | 19.5 ± 0.5 | 2.4 ± 0.4 | 17.3 ± 1.9 |
| Lean mass (g) | 26.0 ± 1.3 | 27.2 ± 0.9 | 24.9 ± 1.4 | 25.8 ± 1.4 |
| Body weight (g) | 30.4 ± 1.4 | 47.7 ± 0.5 | 28.7 ± 1.3 | 43.8 ± 3.2 |
| AUC (ipGTT) | 17,132 ± 918 | 24,707 ± 1,858 | 17,070 ± 2,193 | 26,256 ± 2,919 |
| 24-h food intake(kcal) | 12.5 ± 0.6 | 5.2 ± 0.5 | 11.8 ± 0.6 | 8.3 ± 1.0∗ |
| 24-h water intake (mL) | 3.4 ± 0.2 | 0.8 ± 0.2 | 3.2 ± 0.3 | 1.5 ± 0.2∗ |
| VO2 consumption rate (mL/h/kg) | 4,110 ± 74 | 4,466 ± 69 | 4,050 ± 70 | 4,700 ± 71∗ |
| VCO2 production rate (mL/h/kg) | 3,673 ± 62 | 3,347 ± 56 | 3,661 ± 68 | 3,599 ± 58∗∗ |
| Energy expenditure rate (kcal/h/kg) | 20.3 ± 0.4 | 21.3 ± 0.3 | 20.0 ± 0.3 | 22.5 ± 0.3∗ |
| Respiratory exchange ratio | 0.9 ± 0.0 | 0.7 ± 0.0 | 0.9 ± 0.0 | 0.8 ± 0.0∗∗ |
| Total physical activity (counts/h) | 6,958 ± 475 | 2,722 ± 360 | 4,653 ± 337∗∗∗ | 3,245 ± 188 |
| Liver triglycerides (μmol/g) | 12.8 ± 1.7 | 18.3 ± 4.6 | 11.8 ± 2.4 | 22.1 ± 6.0 |
| Plasma cholesterol (mg/dL) | 81 ± 2.0 | 210 ± 15.0 | 78 ± 3.0 | 198 ± 14.0 |
| HDL (mg/dL) | 71 ± 2.0 | 172 ± 12.0 | 68 ± 3.0 | 155 ± 4.0 |
| LDL (mg/dL) | 74 ± 3.0 | 172 ± 6.0 | 68 ± 4.0 | 170 ± 17.0 |
| Insulin (ng/mL) | 0.56 ± 0.2 (n = 5) | 2.2 ± 0.5# (n = 4) | 0.56 ± 0.1 (n = 5) | 2.7 ± 0.5## (n = 4) |
AUC, area under the curve; HDL, high-density lipoprotein; ipGTT, intraperitoneal glucose tolerance test; LDL, low-density lipoprotein;
Results are expressed as mean ± SEM (n=4 mice/ group).
p values were calculated using an unpaired Student’s t test. ∗p<0.05, ∗∗p<0.01, and ∗∗∗p<0.001 versus villin cre with the same diet; # p<0.05, ##p<0.01 versus RD-fed mice from the same strain.