Table 2. Cost breakdown by laboratory processes.
Costs in USD.
| Item | DNA/RNA
extraction |
DNA/RNA
amplification |
Gel
electrophoresis |
Sequencing * | Sequence
analysis |
Sample
collection |
Total
cost |
|---|---|---|---|---|---|---|---|
| Capital cost | 7.66 | 4.48 | 6.55 | 82.57 | 1.21 | 0.10 | 102.56 |
| Reagents +
consumables |
5.42 | 37.30 | 1.47 | 55.63 | 0 | 1.67 | 101.48 |
| Personnel | 4.68 | 9.36 | 1.87 | 15.44 | 15.44 | 0.47 | 47.27 |
| Utilities | 4.39 | 3.96 | 0.20 | 5.20 | 0.41 | 0.11 | 14.28 |
| Maintenance
cost of equipment |
0.24 | 0.24 | 0.09 | 0.78 | 0.78 | 0.02 | 2.15 |
| Quality
assurance program |
0.40 | 0.80 | 0.16 | 1.32 | 1.32 | 0.04 | 4.03 |
|
Total cost in
USD |
22.79 | 56.14 | 10.34 | 160.94 | 19.16 | 2.41 | 271.78 |
| % Total cost | 8.38 | 20.66 | 3.8 | 59.22 | 7.05 | 0.89 | 100 |
* The most expensive step in HIV drug resistance testing.