Table 3. Cost breakdown of the modified assay.
| Item | DNA/RNA
extraction |
DNA/RNA
amplification |
Gel-
electrophoresis |
Sequencing | Sequence
analysis |
Sample
collection |
Total
cost |
|---|---|---|---|---|---|---|---|
| Capital cost | 7.66 | 4.48 | 6.55 | 82.57 | 1.24 | 0.1 | 102.60 |
| Reagents +
consumables |
5.42 | 19.3 | 1.47 | 35.38 | 0 | 1.67 | 63.24 |
| Personnel | 4.68 | 9.36 | 1.87 | 15.34 | 15.44 | 0.47 | 47.27 |
| Utilities | 4.39 | 3.96 | 0.2 | 5.2 | 0.41 | 0.11 | 14.28 |
| Maintenance
cost of equipment |
0.24 | 0.47 | 0.09 | 0.78 | 0.78 | 0.02 | 2.39 |
| Quality
assurance program |
0.40 | 0.8 | 0.16 | 1.32 | 1.32 | 0.04 | 4.03 |
| Total cost in
USD |
22.79 | 38.38 1 | 10.34 | 140.70 2 | 19.16 | 2.41 | 233.81 3 |
| % Total cost | 9.74 | 16.41 | 4.42 | 60.18 | 8.21 | 1.03 | 100 |
1 Halving reagent volumes at DNA/RNA amplification step reduces the cost of this step from $56.14 to $38.38.
2 Halving reagent volumes at sequencing step reduces the cost of this step from $160.94 to 140.70.
3 Halving reagent volumes at DNA/RNA amplification and sequencing steps reduce the HIV drug resistance test cost to $ 233.81 from $ 271.78.