Skip to main content
. 2020 Jul 29;9:793. [Version 1] doi: 10.12688/f1000research.23379.1

Table 3. Cost breakdown of the modified assay.

Item DNA/RNA
extraction
DNA/RNA
amplification
Gel-
electrophoresis
Sequencing Sequence
analysis
Sample
collection
Total
cost
Capital cost 7.66 4.48 6.55 82.57 1.24 0.1 102.60
Reagents +
consumables
5.42 19.3 1.47 35.38 0 1.67 63.24
Personnel 4.68 9.36 1.87 15.34 15.44 0.47 47.27
Utilities 4.39 3.96 0.2 5.2 0.41 0.11 14.28
Maintenance
cost of
equipment
0.24 0.47 0.09 0.78 0.78 0.02 2.39
Quality
assurance
program
0.40 0.8 0.16 1.32 1.32 0.04 4.03
Total cost in
USD
22.79 38.38 1 10.34 140.70 2 19.16 2.41 233.81 3
% Total cost 9.74 16.41 4.42 60.18 8.21 1.03 100

1 Halving reagent volumes at DNA/RNA amplification step reduces the cost of this step from $56.14 to $38.38.

2 Halving reagent volumes at sequencing step reduces the cost of this step from $160.94 to 140.70.

3 Halving reagent volumes at DNA/RNA amplification and sequencing steps reduce the HIV drug resistance test cost to $ 233.81 from $ 271.78.