TABLE 1.
Primer sets used for site-directed mutagenesis of NF-κB copy number variant LTRs in the pcLGIT/pcLdGIT vector (p911 series) (ATF model)
| LTR variant | Primer paira | Description of primer pair | Sequence of primer (5′–3′) |
|---|---|---|---|
| FHHC (4-κB) | N1992 FP | Inner primers | TGACACAGAAGGGACTTTCTGCTGACACAGAAGGGACTTTCCGCTGGGACTTTCCACTGGGGCGTTCC |
| N1993 RP | AAGTCCCAGCGGAAAGTCCCTTCTGTGTCAGCAGAAAGTCCCTTCTGTGTCAGCAGTCTTTGTAAAACTCCG | ||
| OHHC (3-κB) | N1994 FP | Inner primers | TGACACAGAATCTACTTTTTGCTGACACAGAAGGGACTTTCCGCTGGGACTTTCCACTGGGGCGTTCC |
| N1995 RP | AAGTCCCAGCGGAAAGTCCCTTCTGTGTCAGCAAAAAGTAGATTCTGTGTCAGCAGTCTTTGTAAAACTCCG | ||
| OOHC (2-κB) | N1996 FP | Inner primers | TGACACAGAATCTACTTTTTGCTGACACAGAATCTACTTTTTGCTGGGACTTTCCACTGGGGCGTTCC |
| N1997 RP | AAGTCCCAGCAAAAAGTAGATTCTGTGTCAGCAAAAAGTAGATTCTGTGTCAGCAGTCTTTGTAAAACTCCG | ||
| OOOC (1-κB) | N1998 FP | Inner primers | TGACACAGAATCTACTTTTTGCTGACACAGAATCTACTTTTTGCTTCTACTTTTTACTGGGGCGTTCC |
| N1999 RP | AAGTAGAAGCAAAAAGTAGATTCTGTGTCAGCAAAAAGTAGATTCTGTGTCAGCAGTCTTTGTAAAACTCCG | ||
| OOOO (0-κB) | N2000 FP | Inner primers | TGACACAGAATCTACTTTTTGCTGACACAGAATCTACTTTTTGCTTCTACTTTTTACTTCTACTTTTTAGG |
| N2001 RP | AAGTAGAAGCAAAAAGTAGATTCTGTGTCAGCAAAAAGTAGATTCTGTGTCAGCAGTCTTTGTAAAACTCCG | ||
a
FP, forward primer; RP, reverse primer.