TABLE 3.
Primers used for determination of the proviral integration frequency and chromatin immunoprecipitation-qPCR
| Target region and purpose | Primer pair-probe combination | Primer or probe sequence (5′–3′)a |
|---|---|---|
| Strong-stop DNA for determination of proviral integration frequency | N2208 FP | GATCTGAGCC(T/C)GGGAGCTCTCTG |
| N2209 RP | TCTGAGGGATCTCTAGTTACCAGAGTC | |
| N2210 probe | FAM-CTGCTTAAGCCTCAATAAAGCTTGCCTTGAGTGCT-TAMRA | |
| LTR enhancer for chromatin immunoprecipitation-qPCR | N2493 FP | CCGGAGT(A/T)TTACAAAGACTGCTG |
| N2215 RP | CTGCTTATATGCAGCATCTGAGG | |
| N2492 probe | HEX-CACTGGGGCGTTCCAGG(G/A)GG(A/T)GT-BHQ | |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine; HEX, 6-carboxy-2,4,4,5,7,7-hexachlorofluorescein; BHQ, black hole quencher.