FIG 11.

The HCV life cycle requires proper processing of the NS3-5A polyprotein. Luciferase activity of the reporter wild-type HCV and HCV with the indicated mutations is shown. Cell lysates from Huh 7.5.1 cells transfected with the reporter HCV in vitro transcripts were collected at the indicated time points for measurements. The luciferase activities were normalized against those at the 4-h time point. Values are means ± SEM (n = 3). *, P < 0.05 (determined by t test against the values for the wild type [WT] at the same time points).