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Combinatory mutations that block NS3-4A cleavage block NS5A hyperphosphorylation. (A) Schematics of NS3-5A expression constructs with any two or three mutations at the cleavage junctions. The sites of mutations are numbered and indicated with one-letter amino acid symbols. (B to F) Representative immunoblotting for NS3, NS5A, and NS5A phosphorylated at S2201 (pS2201), S2208 (pS2208), S2211 (pS2211), and S2214 (pS2214) in HEK293T cells transfected with the constructs.
