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. 2020 Sep 15;94(19):e00420-20. doi: 10.1128/JVI.00420-20

FIG 8.

FIG 8

NS5A phosphorylation at serine 2201, 2208, 2211, and 2214 requires NS3-mediated autocleavage at the NS3-4A junction in the native HCV host liver cells. (A) Schematics of four pTM constructs that permit expression of nonstructural proteins in the T7-Huh7 native HCV host liver cells: T1661C (NS3-4A cleavable), T1661R (NS3-4A noncleavable), C1715S (NS4A-4B noncleavable), and C1976F (NS4B-5A noncleavable). (B to F) Immunoblotting for NS3, NS5A, and NS5A phosphorylation at S2201, S2208, S2211, and S2214. (G and H) Summary diagrams. Values are means ± SEM (n = 3). *, P < 0.05 (determined by t test against the values for the wild type [WT]).