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SUMOylated AAV2 capsids display higher thermal stability. (a) Dot blot analysis of AAV2 and n-s-AAV2 particles after incubation at different temperatures. (Inset) The B1 antibody reacts with unassembled VP, while A20 is specific for intact capsids. (b) Quantification of vector genomes of AAV particles shown in panel a. After heat treatment, capsids were incubated with Benzonase to degrade free vector genomes. Packaged vector genomes were quantified by qPCR.
