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AAV activates cellular SUMOylation activity. A549 and HeLa cells were incubated with AAV2 vectors at an MOI of 105. (a) Cell lysates were prepared 4, 8, and 24 h later and analyzed for SUMOylation activity by Western blotting using an antibody specific for SUMO2/3. As a positive control, SUMOylation activity was analyzed after incubating cells with 5 PFU/cell of IAV. (b) Quantification of Western blotting signals shown in panel a, normalized to actin.
