FIG 7.

Evaluation of AAVhum.8 in a humanized mouse model. FAH^−/− humanized liver mice were injected with AAV8 or AAVhum.8 vector expressing scCBh-GFP at a dose of 1e12 vg. Livers were collected 3 weeks postinjection and immunostained for human albumin and GFP. Confocal fluorescence images are shown at 2 mm (A to F) and 50 μm (G to L); magnification, ×40. For each animal, 10 fields of view were examined to count the number of GFP-positive murine and human hepatocytes (M) and the percentage of total human hepatocytes that were GFP⁺ (N). Each data point represents one field of view. n = 3 for AAV8, n = 2 for AAVhum.8.