FIG 5.
MMP-9 inhibition impedes MCV sT-induced cell migration. (A) MCV sT promotes MMP-9-induced cell migration. Scratch assay. Poly-l-lysine-coated 6-well plates were seeded with U2OS cells and incubated with specific MMP-9 inhibitors at predetermined concentrations. Cells were transfected with a vector control, sTWT, or sTLSDm plasmids. After 48 h, a scratch was created, and migration of cells toward the scratch was observed over a 24-h period. The size of the wound was measured at 0 and 24 h and presented as the fold change in panel B. Scratch assays were performed in triplicate. (C) MMP-9 is required for MCC migration. MCV-positive MCC cell lines, MKL-1 and MS-1, were incubated with DMSO or the MMP-9 inhibitors as follows: 9-I (0.2 μM) and 9-II (2 μM) for MKL-1 and 9-I (0.1 μM) and 9-II (1 μM) for MS-1. Cells were then transferred into transwell inserts and allowed to migrate for 48 h. Migrated cells were measured using cell counting kit-8 (CCK-8). Data were analyzed using three biological replicates per experiment (n = 3). Differences between means (P value) were analyzed using a t test with GraphPad Prism software.