FIG 7.
MCV sT LSD induces Snail expression. (A) MCV sT activates the transcription of Snail in an LSD-dependent manner. U2OS cells were transfected with empty vector control, sTWT-, or sTLSDm-expressing plasmids. Cellular RNA was extracted at 48 h posttransfection, and transcript levels were analyzed using the comparative ΔΔCT method (n = 3). (B) Snail protein expression is induced by MCV sT through the LSD. Immunoblot analysis was performed on the cellular lysates and analyzed using Snail-specific antibody. α-Tubulin was used as a measure of equal loading, and the 2T2 antibody was used to confirm MCV sT wild-type and mutant expression.