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. 2020 Sep 15;94(19):e00874-20. doi: 10.1128/JVI.00874-20

TABLE 2.

Primers for inserting the NA gene segments into the pHW2000 plasmid

NA or plasmid Forward primer, 5′ to 3′ Reverse primer, 5′ to 3′
N1-WA01 GAAGTTGGGGGGGAGCAAAAGCAGGAGTTTAAAATGAATC GGTTATTAGTAGAAACAAGGAGTTTTTTCAACGGAC
N1-MI15 CGACCTCCGAAGTTGGGGGGAGCAAAAGCAGGAGTTTAAAATGAATC CATTTTGGGCCGCCGGGTTATTAGTAGAAACAAGGAGTTTTTTGAAC
N1-BR18 TATTCGTCTCAGGGAGCAAAAGCAGGAGT ATATCGTCTCGTATTAGTAGAAACAAGGAGTTTTTT
N2-KA17 CGACCTCCGAAGTTGGGGGGGAGCAAAAGCAGGAGTG CATTTTGGGCCGCCGGGTTATTAGTAGAAACAAGGAG
pHW2000 CCTTGTTTCTACTAATAACC CCTGCTTTTGCTCC