FIG 1.

SP-2509 inhibits viral infection. (A and B) MTT cell proliferation assays (Abcam) were carried out to measure the cytotoxic effects of SP-2509 (A) and OG-L002 (B) on MRC-5 and Vero cells. Cells were incubated in the presence of indicated concentrations of inhibitor for 24 h before conducting the assay according to the manufacturer’s protocol. Data represent the mean of biological triplicate experiments with standard deviation. (C and D) Effects of SP-2509 (C) and OG-L002 (D) on HSV-1 viral yield were determined by infecting 1 × 10⁶ MRC-5 cells with HSV-1 at an MOI of 10 in the presence of the indicated concentrations of inhibitor. Virus was harvested 24 hpi and titers were determined by plaque assay on Vero cells. Data represent the means of biological duplicate experiments with standard deviations. (E) One million MRC-5 cells were infected at an MOI of 0.1 or 10 PFU/cell in the presence or absence of SP-2509 (16 μM) and virus was collected at indicated times. Viral yield was measured via plaque assay on Vero cells. All values represent the means of biological duplicate experiments with standard deviations. Data points without error bars represent reproducible data and error bars that were smaller than the symbol used to represent the data point.