FIG 2.

Effects of LSD1 inhibition on viral protein expression. (A) Western blot of whole-cell lysates collected from SP-2509 treated (16 μM) or untreated MRC-5 cells mock infected or infected with strain KOS and harvested at 2, 4, and 6 hpi. Antibodies are indicated on the right and the viral gene class is indicated on the left. (B) Experiments were carried out as in (A) except that cells were treated with or without OG-L002 (60 μM), infected with strain KOS, and harvested at the indicated times. All infections were carried out at an MOI of 10 PFU/cell and for all inhibitor treatments cells were pretreated with inhibitor for 1 h prior to infection and throughout infection. Average fold change in protein expression (+inhibitor/−inhibitor) was determined from biological duplicate experiments and error bars represent standard deviations.