FIG 3.

SP-2509 inhibits viral gene expression. MRC-5 cells were infected at an MOI of 10 PFU/cell with strain KOS in the presence or absence of SP-2509 (16 μM) and RNA-seq was carried out on total mRNA at 3 and 6 hpi. Sequencing reads were mapped to the human and HSV-1 genome and the percentage of RNA-seq reads that mapped to the viral genome was determined. Data represent the means from biological duplicate experiments with standard deviations. For the 6 hpi time points, results from replicate experiments were identical. Therefore, error bars are not shown on the bar graph. The significance of differences observed in the presence of SP-2509 were determined using a Student’s t test with a statistical significance cutoff at 0.05 (n.s, not significant; ****, P < 0.0001).