FIG 6.

SP-2509 blocks HSV-1 DNA replication. (A and B) MRC-5 cells were infected at an MOI of 0.1 (A) or 10 (B) PFU/cell with strain KOS in the presence or absence of SP-2509 (16 μM). DNA was collected at the indicated times to create a replication curve. Viral genome number was determined by real-time PCR. Error bars represent the means with standard deviations. Data points without error bars represent reproducible data and error bars that were smaller than the symbol used to represent the data point. (C) MRC-5 cells were infected at an MOI of 10 PFU/cell with strain KOS in the presence or absence of SP-2509 (16 μM). EdC was added at 4 hpi, and at 6 hpi cells were fixed. Viral genomes were covalently attached to a fluorophore (vDNA) and probed with antibodies specific to ICP4. Scale bars, 5 μm. Green and red trace of merge (RG) panel is shown at right.