FIG 7.

SP-2509 treatment has no effect on viral genome entry into the nucleus, but results in an increased number of replication compartments per cell. (A) MRC-5 cells were infected with KOS-EdC at an MOI of 10 PFU/cell in the presence or absence of SP-2509 (16 μM) and fixed at 2.5 hpi. Input viral DNA was covalently attached to Alexa Fluor 488 and imaged. The number of input viral genomes per cell was counted. (B) MRC-5 cells were infected with strain KOS at an MOI of 10 PFU/cell in the presence or absence of SP-2509 (16 μM). EdC was added at 4 hpi, and at 6 hpi the cells were fixed. EdC-labeled replicated viral DNA was covalently attached to Alexa Fluor 488 and imaged. The number of viral replication compartments per cell was counted. Horizontal lines in the scatter dot plot represent the means with standard deviations. Significance was determined using a Student’s t test with a statistical significance cutoff of 0.05 (n.s, not significant; ****, P < 0.0001).