Table 2.
Morpholino (MO) Dose per Microinjection
| Diameter of drop (μm) | Radius of drop (μm) | Volume (pL) 4/3πR3 | MO Dose (ng) of 0.5mM = 4.15ng/nl | MO Dose (ng) of 2mM = 16.6ng/nl |
|---|---|---|---|---|
| 1 | 0.5 | 0.52 | 0.00 | 0.01 |
| 2 | 1 | 4.19 | 0.02 | 0.07 |
| 3 | 1.5 | 14.13 | 0.06 | 0.23 |
| 4 | 2 | 33.49 | 0.14 | 0.56 |
| 5 | 2.5 | 65.42 | 0.27 | 1.09 |
| 6 | 3 | 113.04 | 0.47 | 1.88 |
| 7 | 3.5 | 179.50 | 0.74 | 2.98 |
| 8 | 4 | 267.95 | 1.11 | 4.45 |
| 9 | 4.5 | 381.51 | 1.58 | 6.33 |
| 10 | 5 | 523.33 | 2.17 | 8.69 |
| 11 | 5.5 | 696.56 | 2.89 | 11.56 |
| 12 | 6 | 904.32 | 3.75 | 15.01 |
| 13 | 6.5 | 1149.76 | 4.77 | 19.09 |
| 14 | 7 | 1436.03 | 5.96 | 23.84 |
| 15 | 7.5 | 1766.25 | 7.33 | 29.32 |