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. 2020 Sep 16;11:4677. doi: 10.1038/s41467-020-18453-1

Fig. 4. DENR eIF2D double mutants resemble ATF4 mutants.

Fig. 4

a Schematic of developmental transitions during the Drosophila life cycle. b Lethal phase analysis for control, eIF2D, DENR, DENR eIF2D double, and crc animals. Developmental stages are color-coded as shown in the schematic in a. Star symbol indicates animals that arrest during metamorphosis as cryptocephalic pupae. n = 150 for each genotype. c–f Analysis of pupal morphology in dissected control (Canton S), DENR, DENR eIF2D/TM3, and crc mutant animals. Solid arrowheads indicate the degree of wing extension and outlined arrowheads indicate the extent of leg extension. An extended analysis of lethal phase and animal morphology can be found in Supplementary Information (Extended analysis of lethal phase and animal morphology). Data in c–f are representative images collected from two independent biological experiments with ten animals in each trial. Scale bars are 500 μm.