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. 2020 Sep 16;11:4653. doi: 10.1038/s41467-020-18502-9

Fig. 1. HSD-fed Ras/Src-animals exhibit systemic muscle wasting.

Fig. 1

ras1G12V;csk−/− third-instar larvae raised on CD (a) or HSD (b). Transformed tissue is labelled with GFP (green). Secondary tumours are observed in a subset of animals (arrowheads in b). Scale bar, 500 μm. a′, b′ Matching dissected eye epithelial tissue stained with DAPI (red). Scale bar, 250 μm. Nile Red (red) and DAPI (blue) staining of dissected fat body tissue from ras1G12V;csk−/− third-instar larvae fed a CD (c) or HSD (d). Scale bar, 100 μm. F-actin (red) staining of dissected larval body-wall muscle tissue from ras1G12V;csk−/− third-instar larvae raised on CD (e) or HSD (f). Scale bar, 100 μm. LacZ third-instar larvae raised on CD (g) or HSD (h). LacZ-expressing eye tissue is labelled with GFP (green). Scale bar, 500 μm. g′, h′ Matching dissected eye epithelial tissue stained with DAPI (red). Scale bar, 250 μm. Nile Red (red) and DAPI (blue) staining of dissected fat body tissue from LacZ third-instar larvae fed a CD (i) or HSD (j). Scale bar, 100 μm. F-actin (red) staining of dissected larval body-wall muscle tissue from ras1G12V;csk−/− third-instar larvae fed a CD (k) or HSD (l). Scale bar, 100 μm. m Matching body-wall muscle wasting quantification. X-axis represents the percentage of individual animals scoring in each category denoted ‘none', ‘minor', ‘moderate' or ‘strong'.