Skip to main content
. 2020 Sep 16;11:4678. doi: 10.1038/s41467-020-18276-0

Fig. 6. Inhibition of FOXM1 suppresses immune-cell response and inhibits wound healing in vivo.

Fig. 6

a Schematic of in vivo wound-healing assay. CD1 (non-diabetic) mice were wounded and treated topically with either the FOXM1 inhibitor FDI-6 or vehicle every other day for 8 days. b Representative images of wounded skin after topical treatment with either vehicle or FDI-6 at 0, 2, 4, 6, and 8 days after wounding. c Percent of wound area at each time following vehicle or FDI-6 treatment relative to the original wound area. Quantification of wound areas in n = 6 (Veh) and 8 (FDI-6) wounds per group were performed with Fiji software. Data presented as mean ± SEM. *P = 0.042 (two-tailed unpaired Student’s t test). d H&E staining of day 4 wounds demonstrating decreased immune cell infiltrates in FDI-6 treated wounds compared to vehicle control wounds. e Representative pictures of vehicle and FDI-6 treated wounds at day 4 show basal keratin marker K5, and neutrophil marker MPO. Treatment of wounds with FDI-6 resulted in decreased neutrophils compared to vehicle treated wounds. n = 3 animals per group examined over two independent experiments. Data presented as mean ± SD. ***P = 0.0005 (two-tailed unpaired Student’s t test). White arrows indicate the wound edge of the migrating epithelial tongue. Scale bar = 100 µm.