Fig. 1. Structure determination of HKU1 HE by single-particle analysis.
a Representative motion-corrected electron micrograph of HKU1 HE embedded in vitreous ice. Scale bar = 10 nm. b Representative reference-free 2D class averages. c Orthogonal views of the HKU1 HE EM density (coloured by subunit). d Cartoon representation of the atomic model of the dimeric HKU1 HE complex. e EM density (blue mesh) zoned 2 Å around an α-helix comprising residues 112–132. f EM density (blue mesh) zoned 2 Å around a β-sheet comprising residues 181–188, 219–224 and 243–248.