Table III.
Ion PGM versus Sanger sequencing results for 9 esophageal cancer samples.
| # | Sample ID | Pathological diagnosis | Gene | Mutation AA | Ion PGM Variant Frequency (%) | Sanger result Consistent? |
|---|---|---|---|---|---|---|
| 1 | 7 | ESCC | TP53 | p.R175H | 47.01 | YES |
| 2 | 39 | EAC | TP53 | p.A159V | 30.53 | YES |
| 3 | 34 | EAC | TP53 | p.C176F | 39.57 | YES |
| 4 | 3 | ESCC | TP53 | p.R175H | 35.16 | YES |
| 5 | 49 | ESCC | TP53 | p.H179R | 20.20 | YES |
| 6 | 33 | EAC | PIK3CA | p.E542K | 11.14 | YES |
| 7 | 45 | ESCC | TP53 | p.R342* | 66.92 | YES |
| 8 | 47 | ESCC | TP53 | p.C275Y | 12.92 | YES |
| 9 | 8 | ESCC | TP53 | p.R342* | 14.4 | YES |
*
Nonsense mutations resulting in STOP codon; EAC: esophageal adenocarcinoma; ESCC: esophageal squamous cell carcinoma.