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. 2020 Sep 1;5(36):23237–23246. doi: 10.1021/acsomega.0c02991

Figure 2.

Figure 2

CircRIMS is a true circRNA. (a) Schematic illustration of circRIMS with detection and expression primers. The convergent primers DetF and DetR faced each other across the predicted junction site of circRIMS, while the divergent primers expF and expR faced away from each other. The entire circRIMS sequence comprises 630 nucleotides. (b) CircRIMS transcriptional regions on chromosome 6. The circRIMS originated from chr6: 72,295,937–72,333,835 (hg38), which contains 3 exon and 2 intron regions, and the junction site is located between the first intron and the last exon. (c) Agarose gel of RT-qPCR product using detection primers (DetF and DetR) for circRIMS. The PCR product showed a single band between 150 and 200 bp was visualized on a 3% agarose gel. (d) Agarose gel of RT-qPCR product using expression primers (expF and expR) for circRIMS. The PCR product showed a single band between 500 and 750 bp was visualized on a 1% agarose gel.