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. 2020 Sep 2;5(36):23118–23128. doi: 10.1021/acsomega.0c02893

Figure 2.

Figure 2

Uptake of GDVs is dependent on the GDV surface protein integrity and CD98 expression level in the HepG2 cells. (A) 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) cell viability assay on the HepG2 cells after incubation with GDVs for 24 h (the concentration of GDVs was 100 μg/mL; the values represent means ± standard deviation (SD). Data are representative of n = 6 determinations). Uptake of GDVs was (B) concentration-dependent and (C) time-dependent for the first hour and reached a plateau after 2 h. For (B) GDVs were incubated with the HepG2 cells for 6 h and for (C) HepG2 cells were incubated with GDVs at a concentration of 100 μg/mL; the values represent means ± SD. Data are representative of n = 3 determinations. (D–F) illustrate the uptake of GDVs as a function of the CD98 level of expression. (D) Lowest level of CD98 as the downregulation was conducted by CD98 siRNA using oligofecatmine, (E) basal level of CD98 expression on the HepG2 cell, (F) highest level of expression of CD98 in the HepG2 cells (stimulation by 10 μg/mL of lipopolysaccharide (LPS) for 24 h).