Figure 4.

High-throughput methods to identify lung tropic nanomaterials can be used to treat CF. (a) High-throughput barcoding is a novel method of identifying LNPs that efficiently deliver functional nucleic acids in animal models to different tissues. For example, (b) intratracheally delivered LNPs that target epithelial cells in the airway lumen can be identified for treatment of CF. (c) The FIND system requires LNPs to functionally deliver their payload to a target cell, escape the endosome, and translate Cre protein that translocates to the nucleus to facilitate tdTomato expression. LNP, lipid nanoparticle.