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. 2020 Jul 25;412(25):6875–6886. doi: 10.1007/s00216-020-02818-y

Fig. 3.

Fig. 3

Comparison of positive ion mode MALDI and MALDI-2-MSI signal intensity maps with underlying molecular content on the example of PC(34:1). a Schematic depiction of the molar distribution of PC(34:1) in the white matter (WM) and molecular layer (ML) based on quantitative nano-HPLC-ESI-MS analysis after laser microdissection and solid-liquid extraction. bd Signal intensity distribution of [PC(34:1) + H]+ (b), [PC(34:1) + Na]+ (c), and [PC(34:1) + K]+ (d) measured from glass slide with DHAP as a matrix in positive ion mode at 20 μm pixel size using conventional MALDI (right) and MALDI-2 (left)