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. 2020 Jul 25;412(25):6875–6886. doi: 10.1007/s00216-020-02818-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Comparison of negative ion mode MALDI and MALDI-2-MSI signal intensity maps with underlying molecular content on the example of PE(34:1). a Schematic depiction of the molar distribution of PE(34:1) in the white matter (WM) and molecular layer (ML) based on quantitative nano-HPLC-ESI-MS analysis after laser microdissection and solid-liquid extraction. b Signal intensity distribution of [PE(34:1)-H]⁻ measured from glass slide with DHAP as a matrix in negative ion mode at 20 μm pixel size using conventional MALDI (right) and MALDI-2 (left)