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Table 1.
Composition and gradient of eluent used in nano-HPLC-ESI-MS
| t/min | B/% |
|---|---|
| − 30 | 5 |
| 2 | 5 |
| 27 | 30 |
| 35 | 100 |
| 49 | 100 |
| 59 | 5 |