Fig. 7.

High-performance thin layer chromatogram showing: (a) GbE P, Q, R, S, T, U, W, and 1A (Lanes 1–8); Ginkgo biloba leaf (Lanes 9 and 10); Sophora japonica flower (Lanes 11 and 12), S. japonica fruit (Lanes 13 and 14); NIST standard GbE SRM 3247 (Lane 15); genistein (Lane 16); cffeic acid, hyperoside, rutin, and chlorogenic acid (Lanes 17 and 18). Mobile phase (System 1): ethyl acetate: acetic acid: formic acid: water (10/1.1/1.1/2.6) @35–40% humidity. Visualized at 365 nm (b) Lane assignments same as in (a). Visualized with Natural Product Reagent + polyethylene glycol @365 nm. (c) Lane assignments for 1–15 same as in (a) except quercetin, genistin, and genistein (Lane 16); isorhamnetin, caffeic acid, rutin, hyperoside, chlorogenic acid (Lane 17), kaempferol (Lane 18). Mobile phase (System 2): toluene:ethyl acetate: formic acid: (7/3/1) @35–40% humidity. Visualized at 365 nm. (d) Lane assignments as in (c). Visualized with Natural Product Reagent + Polyethylene glycol @365 nm